M.D. MSc.
Nguyen Thuy Loan Chi

Title: Blood Stream Infection at FV Hospital from 2022-2025: Rate and microorganism detection via culture-based and PCR-based method

                                   Nguyen T. L.Chi, Nguyen T.Hang, Le T. Loan, Friend Maviza

Abstract

Bloodstream infections (BSIs) are a significant cause of morbidity and mortality worldwide, particularly with the rising prevalence of antimicrobial resistance. Although conventional blood culture (BC) remains the diagnostic gold standard, its long turnaround time can delay targeted antimicrobial therapy. Rapid molecular diagnostics, such as the FilmArray Blood Culture Identification (BCID) panel, enable earlier pathogen identification and resistance gene detection, potentially improving clinical outcomes. Methods: A cross-sectional study was conducted at FV Hospital between 2022 and 2025 to assess the rate of BSIs, characterize pathogen distribution, and evaluate the performance of the BCID2 assay. Positive blood cultures were processed using standard microbiological methods, and BCID2 testing was performed within one hour after positivity was flagged. Results: The overall incidence of BSIs was approximately 10%. The most frequently identified pathogens were Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus. A total of 510 BCID2 tests were analyzed. High concordance (85.7%) was observed between BCID2 and conventional BC results in monomicrobial infections, while discordance—primarily due to organisms not included in the BCID panel—accounted for most mismatches. In polymicrobial infections, concordance decreased to 46.8%, with some organisms detected only by conventional methods. However, limitations were noted in mixed infections, where low bacterial load or assay constraints may contribute to false-negative results. Conclusions: BSIs at FV Hospital are largely driven by Gram-negative bacteria. The BCID panel provides rapid and clinically useful identification of pathogens, facilitating earlier optimization of therapy. Nevertheless, its limitations in polymicrobial and off-panel cases underscore the continued importance of conventional culture methods in comprehensive BSI management.Top of Form

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Biography of the presenting author

Dr. Nguyen Thuy Loan Chi is a Clinical Biologist with an academic and professional background in medicine and laboratory sciences. She completed her medical degree at the age of 24 at Pham Ngoc Thach University of Medicine. She further pursued advanced studies, earning Master’s degrees from the University of Louvain in Belgium and Paris Descartes University in France. After graduating, she served as a lecturer at Pham Ngoc Thach University of Medicine for five years. Since 2011, she has been working in the Laboratory at FV Hospital, where she specializes in medical diagnostic laboratory.

Presenting author details

Full name: Nguyen Thuy Loan Chi

Contact number: 0975435333

Email: chi4.nguyen@fvhospital.com

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Website: https://www.fvhospital.com/

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Category: Oral presentation